List of Publications

Walter Steffen Lab

zu Publikationsliste der Tierphysiologie

  • Steffen, W. & Fuge, H. (1982) Dynamic changes in autosomal spindle fibres during prometaphase in crane fly spermatocytes. Chromosoma 87: 363-371
  • Fuge, H., Bastmeyer, M. & Steffen, W. (1985) A model for chromosome movement based on lateral interaction of spindle microtubules. J. Theoret. Biol. 115: 391-399
  • Steffen, W. (1985) The 3-dimensional architecture of chromosome fibres in crane fly: Amphitelic autosomal univalents in late prometaphase. Europ. J. Cell Biol. 64 supp. 7
  • Steffen, W. & Fuge, H. (1985) Three-dimensional architecture of chromosome fibres in crane fly: amphitelic autosomal univalents in late prometaphase. Cytobios 43: 199-212
  • Steffen, W., Fuge, H., Dietz, R., Bastmeyer, M., & Müller, G. (1986) Aster-free spindle pole in insect spermatocytes: Evidence for chromosome induced spindle formation? J. Cell Biol. 102: 1679-1688
  • Steffen, W. (1986) Three-dimensional architecture of chromosome fibres in crane fly: co-oriented autosomal bivalents and amphitelic sex univalents. Chromosoma 94: 107-114
  • Steffen, W. (1986) Relationship between the arrangement of microtubules and chromosome behaviour of syntelic autosomal univalents during prometaphase in crane fly spermatocytes. Chromosoma 94: 412-418
  • Bastmeyer, M., Steffen, W., & Fuge, H. (1986) Immunostaining of spindle components in tipulid spermatocytes using a serum against pericentriolar material. Europ. J. Cell Biol. 42: 305-310
  • Linck, R.W., Goggin, M.J., Norrander, J.M., & Steffen, W. (1987) Characterization of antibodies as probes for structural and biochemical studies of tektins from ciliary and flagellar microtubules. J Cell Sci. 88, 453-466
  • Linck, R.W., Steffen, W., & Goggin, M.J. (1987) Tektins in flagellar doublet microtubules and basal bodies. J. Cell Biol. 105, Pt2 95a
  • Steffen, W. & Linck, R.W. (1987) Tektin-like polypeptides in cultured mammalian cells. J. Cell Biol. 105, Pt2 123a
  • Steffen, W. & Linck, R.W. (1988) Evidence for tektins in centrioles and axonemal microtubules. Proc. Natl. Acad. Sci. (USA) 85, 2643-2647
  • Steffen, W. & Linck, R.W. (1988) Are tektins, intermediate filament-like proteins, part of axonemal microtubules, basal bodies and centrioles? Cell Motil. Cytoskel. 10, 346-347
  • Steffen, W. & Linck, R.W. (1988) Tektin-like polypeptides in mammalian cells. Cell Motil. Cytoskel. 11, 209
  • Linck, R.W., Amos, L.A., Amos, W.B., Norrander, J.M., Steffen, W., & Stephens, R.E. (1988) Tektins: Proteins forming integral filaments within certain microtubules. Cell Motil. Cytoskel. 11, 207-208
  • Steffen, W. & Linck, R.W. (1989) Tektins in ciliary and flagellar microtubules and their association with other cytoskeletal systems. In: Cell Movement, vol. 2, Kinesin, Dynein and Microtubule Dynamics. eds. FD Warner, JR McIntosh. (Alan Liss), pp 67-81
  • Steffen, W. & Linck, R.W. (1989) Multiple Immunoblot: A sensitive technique to stain proteins and detect multiple antigens on a single 2-D replica. Electrophoresis 10, 714-718
  • Steffen, W. & Linck, R.W. (1989) Relationship between tektins and intermediate filament proteins: an immunological study. Cell Motil. Cytoskel. 14, 359-371
  • Steffen, W., Fajer, E., Kim, C. & Linck, R.W. (1989) A multiple immunoblot technique used to analyze tektins on 2-D IEF/SDS-PAGE replicas and to study their relationship with intermediate filament proteins. J. Cell Biol. 109 Pt2, 307a
  • Steffen. W. & Linck, R.W. (1990) Evidence for a tektin-related component during mitotic and meiotic cell division. J. Cell Biol. 111 Pt2, 280a
  • Steffen, W. & Linck, R.W. (1990) The presence of a tektin-related component in centrosomes of Spisula solidissima. J. Cell Biol. 111 Pt2, 181a
  • Steffen, W. & Linck, R.W. (1992) Evidence for a non-tubulin spindle matrix and spindle components immunologically related to tektin filaments. J. Cell Sci. 101, 809-822
  • Steffen, W. & Fajer, E.A., & Linck, R.W. (1993) Centrosomal components immuno-related to tektins from flagellar microtubules. Molec. Biol. Cell 4, 395a
  • Steffen, W., Fajer, E., & Linck, R.W. (1994) Centrosomal components immunologically related to tektins from ciliary and flagellar microtubules. J. Cell Sci. 107, 2095-2105
  • Killisch, I., Beug, H., Wiche, G., & Steffen, W. (1995) Charaterization of cytoplasmic dynein in differentiating chicken erythroblasts. Europ. J. Cell Biol. Suppl. 91
  • Steffen, W., Hodgkinson, J.L., & Wiche, G. (1996) Immunogold-localisation of the intermediate chain within the protein complex of cytoplasmic dynein. J. Struct. Biol. 117, 227-235
  • Hodgkinson, J.L. & Steffen, W. (1997) The effect of tetradecyl sodium sulfate on protein mobility and antigen detectability in sodium dodecyl sulfate polyacrylamide gel electrophoresis. Electrophoresis 18, 1955-1959
  • Steffen, W., Karki, S., Vaughan, K.T., Vallee, R.B., Holzbaur, L.E., Weiss, D.G., and Kuznetsov, S.A. (1997) The 74-kD intermediate chain of cytoplasmic dynein is involved in binding the motor to membranous organelles. Molec. Biol. Cell 8, 2077-2088.
  • Steffen, W., Langford, G.M., Weiss, D.G., and Kuznetsov, S.A. (1997) Inhibition of microtubule-dependent, minus-end directed transport of axoplasmic organelles by a dynein intermediate chain-specific antibody. Biol. Bulletin. 193, 221-222.
  • Steffen, W., Hodgkinson, J.L., Weiss, D.G., & Kusnetzov, S.A. (1997) The 74-kD intermediate chain, a regulatory component of cytoplasmic dynein? Biophys. J. 72, A236.
  • Kuznetsov, S.A., Weiss, D.G., Hodgkinson, J.L., & Steffen W. (1997) The 74-kD intermediate chain of cytoplasmic dynein is involved in binding the motor to membranous organelles. Europ. J. Cell Biol.72 (Suppl. 43) 63.
  • Weiss, D.G. and Steffen, W. (1998) Lichtmikroskopie heute: Die lebende Zelle als Meßküvette. BIOspektrum 4(5), 59-67. (
    • pdf-file)
  • Nilsson, H., Steffen, W., Wallin, M., and Palazzo, R.E. (1998) In vitro reconstituion of pigment aggregation in fish melanophores. Molec. Biol. Cell 9, 405a
  • Kuznetsov, S.A., Steffen, W., Vaisberg, E.A., and Palazzo, R.E. (1998) Cytoplasmic dynein is required for spindle formation but not for aster formation in cytoplasmic extracts of Spisula oocytes. Molec. Biol. Cell 9, 280a.
  • Weiss, D.G., Maile, W.,  Wick, R.A., and Steffen, W. (1999) Video microscopy. In: Light Microscopy in Biology. A practical approach. (ed.) A.J. Lacey, Oxford University Press, pp 73-149
  • Palazzo, R., Vaisberg, E., Langford, G., Weiss, D.G., Kuznetsov, S. and Steffen, W. (1999) Spindle assembly in cytoplasmic extracts of Spisula solidissima oocytes. J. Cell Sci. 112, 1291-1302.
  • Steffen, W. and Weiss, D.G. (1999) Biological Imaging. Ein Bild sagt mehr als 1000 Worte... BIOspektrum 5(2), 118-142(  pdf-file)
  • Steffen, W., Kuznetsov, S.A.., Weiss, D.G., Vaisberg, E.A., and Palazzo, R.E. (1999) Cytoplasmic dynein is required for spindle formation but not for aster formation in cytoplasmic extracts of Spisula oocytes. Europ. J. Cell Biol. 78, 54.
  • Stüwe, S., Gramowski, A., Steffen, W., and Weiss, D.G. (1999) Characterizion of cell types and architecture in primary embryonic murine spinal cord culture on multielectrode arrays. Europ. J. Cell Biol.78, 79

    • Palazzo, R.E., Vaisberg, E.A., Weiss, D.G., Kuznetsov, S.A. & Steffen, W. (1999) Dynein is required for spindle assembly in cytoplasmic extracts of Spisula solidissima oocytes. J. Cell Sci. 112, 1292-1302.
  • Rogers, S.L., Karcher, R.L., Roland, J.T., Minin, A.A., Steffen, W., & Gelfand, V.I. (1999) Regulation of melanosome movement by myosin-V throughout the cell cycle. J. Cell Biol. 146, 1265-1275
  • Steffen, W. & Hodgkinson, J.L. (1999) Site-directed antibodies as tools for investigating structure and function of cytoskeleton proteins. Meth. Molec. Biol., vol. Cytoskeleton Methods and Protocols, ed. R.H. Gavin, Humana Press (in press)
  • Steffen, W. & Hodgkinson, J.L. (1999) Direct labeling of components in protein complexes by immuno-electron microscopy. Meth. Molec. Biol., vol. Cytoskeleton Methods and Protocols, ed. R.H. Gavin, Humana Press (in press)
  • Steffen, W. (1999) Tat-mediated delivery of antibodies to cultured cells. Meth. Molec. Biol., vol. Cytoskeleton Methods and Protocols, ed. R.H. Gavin, Humana Press (in press)
  • Suddith, A.W., Vaisberg, E.A., Kuznetsov, S.A., Steffen, W., Rieder, C.L., & Palazzo, R.E. (1999) Centriol duplication, centrosome maturation and spindle formation in lysates of Spisula solidissima oocytes. Meth. Molec. Biol., vol. Cytoskeleton Methods and Protocols, ed. R.H. Gavin, Humana Press (in press)
  • Weiss, D.G., Tychinsky, V.P., Steffen, W., & Budde, A. (1999) Digital microscopy techniques for the study of living cytoplasm. In: Image Analysis in Biology, 2nd. ed., D.-P. Häder (ed.), CRC Press, Boca Raton (in press)
  • Steffen, W., Kuznetsov, S.A., Holzbaur, E.L.F., Langford, G.M., Weiss, D.G., & Palazzo, R.E. (1999) The dynein-dynactin complex is required for cytaster assembly but not for centrosome-dependent aster formation in vitro. Molec. Biol. Cell (in press)
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