Grundlagenforschung Ersatz zu Tierversuchen...

Funktionelles Screening von Neuropharmaka mit neuronalen Zellkulturen

"Das Gehirn ist dynamisch in zelluläre Grundgesamtheiten organisiert, ... die die funktionellen Einheiten darstellen."
(GM Edelmann, 1987)

Methoden

Publikationen

• Gramowski, A., Jügelt, K., Stüwe, S., Schulze, R., McGregor, G.P., Wartenberg-Demand, A., Loock, J., Schröder, O., Weis, D.G.: Functional Screening of Traditional Antidepressants with Primary Cortical Neuronal Networks Grown on Multielectrode Neurochips. Eur J Neurosci. 1-11 (2006)
• Gramowski A, Stüwe S, Jügelt K, Schiffmann D, Loock J, Gross GW, Schröder O, Weiss DG.: Detecting neurotoxicity through electrical activity changes of neuronal networks on microelectrode neurochips. ALTEX. 23: 410-415 (2006)
• Gramowski, A., Jügelt, K., Weiss, D.G., Gross, G.W.: Substance identification by quantitative characterization of oscillatory activity in murine spinal cord networks on microelectrode arrays. Eur J Neurosci. 19: 2815-2825 (2004)
• Gross GW, Dian EA, Keefer EW, Gramowski A, Stuewe S.: Long-term functional contact between nerve cell networks and microelectrode arrays. In: Toward Replacement Parts for the Brain: Implantable Biomimetic Electronics as Neural Prostheses. Burger T, Glanzman D (eds.). MIT Press. 177-204 (2005)
• Keefer, E.W., Gramowski, A., Stenger, D.A., Pancrazio, J.J., Gross, G.W.: Characterization of acute neurotoxic effects of trimethylopropane phosphate via neuronal network biosensors. Biosensors & Bioelectronics. 16: 513-525 (2001)
• Loock, J., Stange, J., Mitzner, S., Schmidt, R., Gramowski, A., Schiffmann, D., Weiss, D.G., Keefer, E., Gross, G.W.: Influence of albumine dialysis (MARS) on neuronal network activity in vitro - early results. German Journal of Gastroenterology. 39 (Supplement): 28-32 (2001)
• Keefer, E.W., Gramowski, A., Gross, G.W.: Presynaptic control of synchronized periodic oscillations in neuronal populations. J Neurophysiol. 86: 3030-3042 (2001)
• Gramowski, A., Schiffmann, D., Gross, G.W. Quantification of acute neurotoxic effects of trimethyltin using neuronal networks cultured on microelectrode arrays. NeuroToxicology. 21: 331-342 (2000)
  

• Mikroelektroden-Array-Neurochip
• Gehirnregion-spezifische neuronale Netzwerke in vitro
• Spike Train Analyse