Cell Monitoring System (CMS®)

Biochemical substances are sensitively recognized and processed by living cells, either to provide life-energy or to trigger an adequate cell-type specific response. For on-line monitoring of cellular reactions we develop(ed) different Cell Monitoring Systems (CMSTM)(see figure CMS).

Cell(s) need:
physiological conditions
(nutrition, osmolarity, temp.-range, ..)
Sensors / measurement:
  • non-invasive
  • multi-parametrc
  • on-line
    		•

    To measure the influence of test compounds on living cells we have to measure the cellular output signals. But first of all we have to ensure physiological conditions to keep the cells alive in an environment which is as near as possible to natural or in-vivo conditions.

    We have to guarantee for instance the adequate nutrition, temperature range and osmolarity of used liquids before we (can) apply the test substances.

    If we combine the cells with chemical and physical microsensors we get a Cell Monitoring System. Beside the measurement of the cellular output signals we also have to control the input signals. The measurement has to be non-invasive to avoid a disturbance of the cellular system, it has to be multiparametric (to measure different parameters) and on-line to get information about the reaction kinetics. The more accurate you want to detect the cellular reaction, the more parameter you have to measure.

    The different Cell Monitoring Systems all show a similar setup (see figure below).

    Neurochip mounted in a 40-pin IC socket 
    (rear chip with encapsulation)

    40-pin IC socket with sensorchip for metabolic parameters
    (rear chip with encapsulation and flow injection head)

    They consist of a temperature controlled cell culture chamber with a fluid handling and life support system. The different microsensors are connected via an electronic interface with the data acquisition and control unit. The cells are directly growing on the sensorchip and, if necessary, separated by a semipermeable membrane from the fluid handling system. External sensors are used to control special parameters

    CMS® Applications:

    • biomedical research
    • pharmaceutical drug development
    • bioreactor control
    • environmental monitoring
    • military warning systems
    Measurements and further information can be found in:

    Werner Baumann, Erik Schreiber, Guido Krause, Simone Stüwe, Angela Podssun, Susanne Homma, Holger Anlauf, Ingo Freund, Ronny Rosner and Mirko Lehmann. Multiparametric neurosensor microchip: Proceedings Eurosensors XVI, Prag, Sept. 2002
    Article in pdf-format (200 kb)

    Baumann, W., Lehmann, M., Bitzenhofer, M., Schwinde, A., Brischwein, M., Ehret, R. und B.Wolf. 1999: Microelectronic sensor system for microphysiological application on living cells, Sensors and Actuators B, B 55, 77-89
    Article in pdf-format

    Ehret, R., W. Baumann, M. Brischwein, A. Schwinde, B. Wolf. 1998. On-line control of cellular adhesion with impedance measurements using interdigitated electrode structures. Medical & Biological Engineering & Computing, 36, 365-370
    Article in pdf-format


    For more information have a look on the entire publication list.

     

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